An accessible database for mouse and human whole transcriptome qPCR primers

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Abstract

Motivation: Real time quantitative PCR (qPCR) is an important tool in quantitative studies of DNA and RNA molecules; especially in transcriptome studies, where different primer combinations allow identification of specific transcripts such as splice variants or precursor mRNA (pre-mRNA). Several softwares which implement various rules for optimal primer design are available. Nevertheless, since designing qPCR primers needs to be done manually, the repeated task is tedious, time consuming and prone to errors.
Results: We used a set of rules to automatically design all possible exon-exon and intron-exon junctions in the Human and Mouse transcriptomes. The resulting database is included as a track in the UCSC genome browser, making it widely accessible and easy to use.
Availability: The database is available from the UCSC genome browser (http://genome.ucsc.edu/), track name “Whole Transcriptome qPCR Primers” for the hg19 (Human) and mm10 (Mouse) genome versions. Batch query is available in:http://www.weizmann.ac.il/complex/compphys/software/Amit/primers/batchqueryqpcrprimers.htm