Introduction | |
QualitySNPng is a software tool for the detection and visualisation of single nucleotide polymorphisms (SNPs) from next generation sequencing data that uses a haplotype-based strategy, does not require a fully sequenced reference genome and works on polyploid species. QualitySNPng builds on the principles of SNP detection as described in the QualitySNP pipeline (Tang et al, BMC bioinformatics 2006,7:438). It is optimised for the analysis of next generation sequencing data and it features: -a user-friendly interface for SNP calling and visualisation -multiple filtering options to handle typical sequencing errors -support for SAM and ACE formatted input files -exportable marker lists for the creation of SNP arrays -visualisation of SNPs per sample (read groups) It runs on Windows, Mac OS X and Linux, and can be used as a stand-alone application with a graphical user interface or as part of a pipeline system like Galaxy. The output data is saved as CSV text files and can be reloaded for later analysis using QualitySNPng, or processed by custom scripts for further analysis. QualitySNPng was written in C++ using the Qt toolkit. For further information please email: qsnpng @ bioinformatics.nl
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